How do serial dilution

Example 1 What is the dilution factor if you add 0. Example 2 If you did the above dilution four times, what would be the final dilution factor? Solution 2 Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume.

Related questions How do you calculate concentration from absorbance? How do you calculate concentration from titration? How do you calculate dilution factor? How to calculate concentration of solution when it's diluted? What are some examples of dilution calculations? What would be the concentration of a solution made by adding mL of water to What would be the concentration of a solution made by diluting In addition, the bacteria might need a longer time than usual to show growth.

Any of these could be a possibility, which means more tests need to be done to completely understand and address the issue and come up with a conclusion. Not Helpful 2 Helpful 4. How can I set up a series of tubes with a dilution such that I end up with 3mL of solution in each tube at the end of the procedure?

If you're using 4 tubes, altogether, after dilution, that's 12mL. It's the 3mL times the number of tubes, which is 4. One quarter of 12mL is 3mL. This means you should have 3mL in each tube if you divide the total amount into the 4 tubes. Not Helpful 24 Helpful 8. If I had 2 ml of a substance and then added 4 ml of a dilution, what would my ratio be?

Not Helpful 1 Helpful 7. Include your email address to get a message when this question is answered. By using this service, some information may be shared with YouTube. Related wikiHows How to. How to. About This Article. Co-authored by:. Co-authors: Updated: June 21, Categories: Chemistry. Article Summary X To do serial dilutions, start by filling several test tubes with 9 milliliters of a dilution liquid, like water. Italiano: Eseguire il Metodo delle Diluizioni Seriali.

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Thank you so much! Rated this article:. Moza Shareefa Jun 6, It was a very helpful during my preparations for a seminar. Katan Ali Oct 8, Anonymous Oct 20, I need a hint Start with a dilution someone else has made. Take 1 mL and put it in 99 mLs of water. Put one mL of a stock into 49 mLs of water. Take 1 mL of that and put it in 49 mLs of water. Repeat 3 more times. Start with a , dilution someone else has made. Starting with a dilution made by a TA, you add 1 mL to 49 mLs of water to get a , dilution.

What was the TA's dilution factor?